Journal of Reproductive Immunology
Volume 86, Issue 2 , Pages 126-132 , November 2010

Performance evaluation of microbead and ELISA assays for follicular G-CSF: a non-invasive biomarker of oocyte developmental competence for embryo implantation

  • Nathalie Lédée

      Affiliations

    • INSERM U782, Université Paris-Sud, UMR-S0782, Clamart F-92140, France
    • Université de Liège, Département universitaire de Gynécologie et d’obstétrique, CHR la Citadelle, Liège, Belgium
    • Corresponding Author InformationCorresponding author at: INSERM U782, Université Paris-Sud, UMR-S0782, Clamart F-92140, France. Tel.: +33 145374450; fax: +33 145374450.
    • ,
  • Carine Munaut

      Affiliations

    • Université de Liège, Département universitaire de Gynécologie et d’obstétrique, CHR la Citadelle, Liège, Belgium
    • ,
  • Valérie Sérazin

      Affiliations

    • Service de Medecine de la Reproduction et de Biologie Moléculaire, CHI Poissy St. Germain, EA-2493 Poissy, France
    • ,
  • Sophie Perrier d’Hauterive

      Affiliations

    • Université de Liège, Département universitaire de Gynécologie et d’obstétrique, CHR la Citadelle, Liège, Belgium
    • ,
  • Letizia Lombardelli

      Affiliations

    • Centre of Excellence for Research, Transfer and High Education DENOTHE of the University of Florence, Department of Internal Medicine-Immuno-allergology Unit, viale Morgagni 85, 50134 Florence, Italy
    • ,
  • Federica Logiodice

      Affiliations

    • Centre of Excellence for Research, Transfer and High Education DENOTHE of the University of Florence, Department of Internal Medicine-Immuno-allergology Unit, viale Morgagni 85, 50134 Florence, Italy
    • ,
  • Robert Wainer

      Affiliations

    • Service de Medecine de la Reproduction et de Biologie Moléculaire, CHI Poissy St. Germain, EA-2493 Poissy, France
    • ,
  • Virginie Gridelet

      Affiliations

    • Université de Liège, Département universitaire de Gynécologie et d’obstétrique, CHR la Citadelle, Liège, Belgium
    • ,
  • Gérard Chaouat

      Affiliations

    • INSERM U782, Université Paris-Sud, UMR-S0782, Clamart F-92140, France
    • ,
  • Francis Frankenne

      Affiliations

    • Université de Liège, Département universitaire de Gynécologie et d’obstétrique, CHR la Citadelle, Liège, Belgium
    • ,
  • Jean Michel Foidart

      Affiliations

    • Université de Liège, Département universitaire de Gynécologie et d’obstétrique, CHR la Citadelle, Liège, Belgium
    • ,
  • Marie-Pierre Piccinni

      Affiliations

    • Centre of Excellence for Research, Transfer and High Education DENOTHE of the University of Florence, Department of Internal Medicine-Immuno-allergology Unit, viale Morgagni 85, 50134 Florence, Italy

Received 11 December 2009 ,Revised 4 May 2010 ,Accepted 20 May 2010.

References 

  1. Aziz N, Nishanian P, Mitsuyasu R, Detels R, Fahey JL. Variables that affect assays for plasma cytokines and soluble activation markers. Clin. Diagn. Lab. Immunol. 1999;6:89–95
  2. de Jager W, Prakken BJ, Bijlsma JW, Kuis W, Rijkers GT. Improved multiplex immunoassay performance in human plasma and synovial fluid following removal of interfering heterophilic antibodies. J. Immunol. Methods. 2005;300:124–135
  3. Dupont NC, Wang K, Wadhwa PD, Culhane JF, Nelson EL. Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: determinations of a panel of nine cytokines in clinical sample culture supernatants. J. Reprod. Immunol. 2005;66:175–191
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  6. Lédée N, Lombroso R, Lombardelli L, Selva J, Dubanchet S, Chaouat G, et al. Cytokines and chemokines in follicular fluids and potential of the corresponding embryo: the role of granulocyte colony-stimulating factor. Hum Reprod. 2008;23:2001–2009
  7. Lédée N, Frydman R, Osipova A, Taieb J, Gallot V, Lombardelli L, Logiodice F, Petitbarat M, Fanchin R, Chaouat G, Achour-Frydman N, Piccinni MP.Levels of follicular G-CSF and interleukin-15 appear as noninvasive biomarkers of subsequent successful birth in modified natural in vitro fertilization/intracytoplasmic sperm injection cycles. http://www.ncbi.nlm.nih.gov/pubmed/20400073 Fertil Steril. 2010 Apr 16. [Epub ahead of print] PMID: 20400073.
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  10. Salmassi A, Schmutzler AG, Huang L, Hedderich J, Jonat W, Mettler L. Detection of granulocyte colony-stimulating factor and its receptor in human follicular luteinized granulosa cells. Fertil. Steril. 2004;81(Suppl. 1):786–791
  11. Salmassi A, Schmutzler AG, Schaefer S, Koch K, Hedderich J, Jonat W, et al. Is granulocyte colony-stimulating factor level predictive for human IVF outcome?. Hum. Reprod. 2005;20:2434–2440
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  13. Yanagi K, Makinoda S, Fujii R, Miyazaki S, Fujita S, Tomizawa H, et al. Cyclic changes of granulocyte colony-stimulating factor (G-CSF) mRNA in the human follicle during the normal menstrual cycle and immunolocalization of G-CSF protein. Hum. Reprod. 2002;17:3046–3052
  14. Young SH, Antonini JM, Roberts JR, Erdely AD, Zeidler-Erdely PC. Performance evaluation of cytometric bead assays for the measurement of lung cytokines in two rodent models. J. Immunol. Methods. 2008;331:59–68

☆ Financial support: This work was supported by the European network of excellence EMBIC (contract 512040) and the french agency of biomedicine.

PII: S0165-0378(10)00098-7

doi: 10.1016/j.jri.2010.05.003

Journal of Reproductive Immunology
Volume 86, Issue 2 , Pages 126-132 , November 2010

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