Journal of Reproductive Immunology
Volume 85, Issue 1 , Pages 63-70 , May 2010

Measurement of cell death by oxidative stress in three-dimensional spheroids from trophoblast and in fragments of decidua tissue

  • Regine-Susanne Theuerkauf

      Affiliations

    • Institut für Zellbiologie, Histologie und Embryologie, Medizinische Universität Graz, Harrachgasse 21, A-8010 Graz, Austria
    • ,
  • Helmut Ahammer

      Affiliations

    • Insitut für Biophysik, Medizinische Universität Graz, A-8010 Graz, Austria
    • ,
  • Monika Siwetz

      Affiliations

    • Institut für Zellbiologie, Histologie und Embryologie, Medizinische Universität Graz, Harrachgasse 21, A-8010 Graz, Austria
    • ,
  • Christine Helige

      Affiliations

    • Institut für Zellbiologie, Histologie und Embryologie, Medizinische Universität Graz, Harrachgasse 21, A-8010 Graz, Austria
    • ,
  • Gottfried Dohr

      Affiliations

    • Institut für Zellbiologie, Histologie und Embryologie, Medizinische Universität Graz, Harrachgasse 21, A-8010 Graz, Austria
    • ,
  • Wolfgang Walcher

      Affiliations

    • Universitätsklinik für Frauenheilkunde und Geburtshilfe, Medizinische Universität Graz, A-8036 Graz, Austria
    • ,
  • José Ramón Palacio

      Affiliations

    • Instituto de Biotecnologia y Biomedicina, Universidad Autónoma de Barcelona, E-08193 Bellaterra, Spain
    • ,
  • Paz Martinez

      Affiliations

    • Instituto de Biotecnologia y Biomedicina, Universidad Autónoma de Barcelona, E-08193 Bellaterra, Spain
    • ,
  • Peter Sedlmayr

      Affiliations

    • Institut für Zellbiologie, Histologie und Embryologie, Medizinische Universität Graz, Harrachgasse 21, A-8010 Graz, Austria
    • Corresponding Author InformationCorresponding author. Tel.: +43 316 380 4234; fax: +43 316 380 9625.

Received 30 October 2009 ,Revised 15 January 2010 ,Accepted 26 January 2010.

References 

  1. Badia R, Iborra A, Palacio JR, Antich M, Martinez P. The effect of oxidative environment on immunosuppressive properties of human seminal plasma. Am. J. Reprod. Immunol. 2008;60:354–360
  2. Buenz EJ, Limburg PJ, Howe CL. A high-throughput 3-parameter flow cytometry-based cell death assay. Cytometry A. 2007;71:170–173
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  4. Grümmer R, Hohn H-P, Denker H-W. Choriocarcinoma cell spheroids: an in vitro model for the human trophoblast. Trophoblast Res. 1990;4:97–111
  5. Guerin P, El Mouatassim S, Menezo Y. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum. Reprod. Update. 2001;7:175–189
  6. Helige C, Ahammer H, Hammer A, Huppertz B, Frank HG, Dohr G. Trophoblastic invasion in vitro and in vivo: similarities and differences. Hum. Reprod. 2008;23:2282–2291
  7. Hoffman RM. Three-dimensional histoculture: origins and applications in cancer research. Cancer Cells. 1991;3:86–92
  8. Iborra A, Palacio JR, Martinez P. Oxidative stress and autoimmune response in the infertile woman. Chem. Immunol. Allergy. 2005;88:150–162
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  10. Khadaroo RG, Kapus A, Powers KA, Cybulsky MI, Marshall JC, Rotstein OD. Oxidative stress reprograms lipopolysaccharide signaling via Src kinase-dependent pathway in RAW 264.7 macrophage cell line. J. Biol. Chem. 2003;278:47834–47841
  11. Kumar HR, Zhong X, Hoelz DJ, Rescorla FJ, Hickey RJ, Malkas LH, et al. Three-dimensional neuroblastoma cell culture: proteomic analysis between monolayer and multicellular tumor spheroids. Pediat. Surg. Int. 2008;24:1229–1234
  12. Lambert B, De Ridder L, De Vos F, Slegers G, de Gelder V, Van de Wiele C, et al. Assessment of supra-additive effects of cytotoxic drugs and low dose rate irradiation in an in vitro model for hepatocellular carcinoma. Can. J. Physiol. Pharmacol. 2006;84:1021–1028
  13. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 1983;65:55–63
  14. Mueller-Klieser W. Three-dimensional cell cultures: from molecular mechanisms to clinical applications. Am. J. Physiol. 1997;273:C1109–C1123
  15. Sies H. Oxidative stress: oxidants and antioxidants. Exp. Physiol. 1997;82:291–295
  16. Verma P, Verma V, Ray P, Ray AR. Formation and characterization of three dimensional human hepatocyte cell line spheroids on chitosan matrix for in vitro tissue engineering applications. In Vitro Cell. Dev. Biol.-Anim. 2007;43:328–337

PII: S0165-0378(10)00038-0

doi: 10.1016/j.jri.2010.01.004

Journal of Reproductive Immunology
Volume 85, Issue 1 , Pages 63-70 , May 2010

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