Journal of Reproductive Immunology
Volume 84, Issue 2 , Pages 193-198 , March 2010

Effect of vascular endothelial growth factor and interleukin-1β on apoptosis in endometrial cell cultures from patients with endometriosis and controls

  • Mariela Bilotas

      Affiliations

    • Instituto de Biología y Medicina Experimental (IBYME), Vuelta de Obligado 2490, C1428ADN Buenos Aires, Argentina
    • Corresponding Author InformationCorresponding author. Tel.: +54 11 47832869; fax: +54 11 47842564.
    • ,
  • Gabriela Meresman

      Affiliations

    • Instituto de Biología y Medicina Experimental (IBYME), Vuelta de Obligado 2490, C1428ADN Buenos Aires, Argentina
    • ,
  • Ricardo Buquet

      Affiliations

    • Departamento de Ginecología, Hospital de Clínicas “José de San Martín”, C1120AAF Buenos Aires, Argentina
    • ,
  • Carlos Sueldo

      Affiliations

    • Centro de Estudios en Ginecología y Reproducción (CEGyR), C1055ABB Buenos Aires, Argentina
    • ,
  • Rosa Inés Barañao

      Affiliations

    • Instituto de Biología y Medicina Experimental (IBYME), Vuelta de Obligado 2490, C1428ADN Buenos Aires, Argentina

Received 17 March 2009 ,Revised 18 November 2009 ,Accepted 1 December 2009.

References 

  1. Abrams JM, White K, Fessler LI, Steller H. Programmed cell death during Drosophila embryogenesis. Development. 1993;117:29–43
  2. Andreu C, Parborell F, Vanzulli S, Chemes H, Tesone M. Regulation of follicular luteinization by a gonadotropin-releasing hormone agonist: relationship between steroidogenesis and apoptosis. Mol. Reprod. Dev. 1998;51:287–294
  3. ASRM . Revised American Society for Reproductive Medicine classification of endometriosis: 1996. Fertil. Steril. 1997;67:817–821
  4. Baek JH, Jang JE, Kang CM, Chung HY, Kim ND, Kim KW. Hypoxia-induced VEGF enhances tumor survivability via suppression of serum deprivation-induced apoptosis. Oncogene. 2000;19:4621–4631
  5. Bergqvist A, Bruse C, Carlberg M, Carlstrom K. Interleukin 1beta, interleukin-6, and tumor necrosis factor-alpha in endometriotic tissue and in endometrium. Fertil. Steril. 2001;75:489–495
  6. Berkkanoglu M, Guzeloglu-Kayisli O, Kayisli UA, Selam BF, Arici A. Regulation of Fas ligand expression by vascular endothelial growth factor in endometrial stromal cells in vitro. Mol. Hum. Reprod. 2004;10:393–398
  7. Bilotas M, Baranao RI, Buquet R, Sueldo C, Tesone M, Meresman G. Effect of GnRH analogues on apoptosis and expression of Bcl-2, Bax, Fas and FasL proteins in endometrial epithelial cell cultures from patients with endometriosis and controls. Hum. Reprod. 2007;22:644–653
  8. Borroni R, Di Blasio AM, Gaffuri B, Santorsola R, Busacca M, Vigano P, et al. Expression of GnRH receptor gene in human ectopic endometrial cells and inhibition of their proliferation by leuprolide acetate. Mol. Cell Endocrinol. 2000;159:37–43
  9. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976;72:248–254
  10. Brusselmans K, Bono F, Collen D, Herbert JM, Carmeliet P, Dewerchin M. A novel role for vascular endothelial growth factor as an autocrine survival factor for embryonic stem cells during hypoxia. J. Biol. Chem. 2005;280:3493–3499
  11. Castellon E, Clementi M, Hitschfeld C, Sanchez C, Benitez D, Saenz L, et al. Effect of leuprolide and cetrorelix on cell growth, apoptosis, and GnRH receptor expression in primary cell cultures from human prostate carcinoma. Cancer Invest. 2006;24:261–268
  12. Castigli E, Arcuri C, Giovagnoli L, Luciani R, Giovagnoli L, Secca T, et al. Interleukin-1beta induces apoptosis in GL15 glioblastoma-derived human cell line. Am. J. Physiol. Cell Physiol. 2000;279:C2043–C2049
  13. Crosignani P, Olive D, Bergqvist A, Luciano A. Advances in the management of endometriosis: an update for clinicians. Hum. Reprod. Update. 2006;12:179–189
  14. Dmowski WP, Ding J, Shen J, Rana N, Fernandez BB, Braun DP. Apoptosis in endometrial glandular and stromal cells in women with and without endometriosis. Hum. Reprod. 2001;16:1802–1808
  15. Donnez J, Smoes P, Gillerot S, Casanas-Roux F, Nisolle M. Vascular endothelial growth factor (VEGF) in endometriosis. Hum. Reprod. 1998;13:1686–1690
  16. El BH, Rath G, Morjani H, Schneider C, Petitfrere E, Antonicelli F, et al. Interleukin-1beta-induced apoptosis through adenylyl cyclase and ERK1/2 inhibition in primary cultured thyroid cells. Biochem. Biophys. Res. Commun. 2006;339:469–476
  17. Garcia-Velasco JA, Arici A, Zreik T, Naftolin F, Mor G. Macrophage derived growth factors modulate Fas ligand expression in cultured endometrial stromal cells: a role in endometriosis. Mol. Hum. Reprod. 1999;5:642–650
  18. Giudice LC, Kao LC. Endometriosis. Lancet. 2004;364:1789–1799
  19. Groothuis PG, Nap AW, Winterhager E, Grummer R. Vascular development in endometriosis. Angiogenesis. 2005;8:147–156
  20. Harada T, Taniguchi F, Izawa M, Ohama Y, Takenaka Y, Tagashira Y, et al. Apoptosis and endometriosis. Front. Biosci. 2007;12:3140–3151
  21. Imai A, Takagi A, Tamaya T. Gonadotropin-releasing hormone analog repairs reduced endometrial cell apoptosis in endometriosis in vitro. Am. J. Obstet. Gynecol. 2000;182:1142–1146
  22. Kosaka N, Sudo N, Miyamoto A, Shimizu T. Vascular endothelial growth factor (VEGF) suppresses ovarian granulosa cell apoptosis in vitro. Biochem. Biophys. Res. Commun. 2007;363:733–737
  23. Kyama CM, Overbergh L, Mihalyi A, Meuleman C, Mwenda JM, Mathieu C, et al. Endometrial and peritoneal expression of aromatase, cytokines, and adhesion factors in women with endometriosis. Fertil. Steril. 2008;89:301–310
  24. Lebovic DI, Bentzien F, Chao VA, Garrett EN, Meng YG, Taylor RN. Induction of an angiogenic phenotype in endometriotic stromal cell cultures by interleukin-1beta. Mol. Hum. Reprod. 2000;6:269–275
  25. Markstrom E, Svensson EC, Shao R, Svanberg B, Billig H. Survival factors regulating ovarian apoptosis—dependence on follicle differentiation. Reproduction. 2002;123:23–30
  26. Marshall JC, Jia SH, Parodo J, Watson RW. Interleukin-1beta mediates LPS-induced inhibition of apoptosis in retinoic acid-differentiated HL-60 cells. Biochem. Biophys. Res. Commun. 2008;369:532–538
  27. McLaren J, Prentice A, Charnock-Jones DS, Smith SK. Vascular endothelial growth factor (VEGF) concentrations are elevated in peritoneal fluid of women with endometriosis. Hum. Reprod. 1996;11:220–223
  28. McNamee JP, Bellier PV, Kutzner BC, Wilkins RC. Effect of pro-inflammatory cytokines on spontaneous apoptosis in leukocyte sub-sets within a whole blood culture. Cytokine. 2005;31:161–167
  29. Mercurio AM, Lipscomb EA, Bachelder RE. Non-angiogenic functions of VEGF in breast cancer. J. Mammary Gland. Biol. Neoplasia. 2005;10:283–290
  30. Meresman GF, Bilotas MA, Lombardi E, Tesone M, Sueldo C, Baranao RI. Effect of GnRH analogues on apoptosis and release of interleukin-1beta and vascular endothelial growth factor in endometrial cell cultures from patients with endometriosis. Hum. Reprod. 2003;18:1767–1771
  31. Meresman GF, Vighi S, Buquet RA, Contreras-Ortiz O, Tesone M, Rumi LS. Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis. Fertil. Steril. 2000;74:760–766
  32. Mori H, Sawairi M, Nakagawa M, Itoh N, Wada K, Tamaya T. Expression of interleukin-1 (IL-1) beta messenger ribonucleic acid (mRNA) and IL-1 receptor antagonist mRNA in peritoneal macrophages from patients with endometriosis. Fertil. Steril. 1992;57:535–542
  33. Nisolle M, Casanas-Roux F, Anaf V, Mine JM, Donnez J. Morphometric study of the stromal vascularization in peritoneal endometriosis. Fertil. Steril. 1993;59:681–684
  34. Nor JE, Christensen J, Mooney DJ, Polverini PJ. Vascular endothelial growth factor (VEGF)-mediated angiogenesis is associated with enhanced endothelial cell survival and induction of Bcl-2 expression. Am. J. Pathol. 1999;154:375–384
  35. Olivares C, Bilotas M, Buquet R, Borghi M, Sueldo C, Tesone M, et al. Effects of a selective cyclooxygenase-2 inhibitor on endometrial epithelial cells from patients with endometriosis. Hum. Reprod. 2008;23:2701–2708
  36. Park A, Chang P, Ferin M, Xiao E, Zeitoun K. Inhibition of endometriosis development in rhesus monkeys by blocking VEGF receptor: a novel treatment for endometriosis. Fertil. Steril. 2004;82:S71
  37. Pupo-Nogueira A, de Oliveira RM, Petta CA, Podgaec S, Dias JA, Abrao MS. Vascular endothelial growth factor concentrations in the serum and peritoneal fluid of women with endometriosis. Int. J. Gynaecol. Obstet. 2007;99:33–37
  38. Ribble D, Goldstein NB, Norris DA, Shellman YG. A simple technique for quantifying apoptosis in 96-well plates. BMC Biotechnol. 2005;5:12-
  39. Ryan IP, Schriock ED, Taylor RN. Isolation, characterization, and comparison of human endometrial and endometriosis cells in vitro. J. Clin. Endocrinol. Metab. 1994;78:642–649
  40. Sampson JA. Peritoneal endometriosis due to menstrual dissemination of endometrial tissue into the peritoneal cavity. Am. J. Obstet. Gynecol. 1927;14:422–469
  41. Simon C, Pellicer A, Polan ML. Interleukin-1 system crosstalk between embryo and endometrium in implantation. Hum. Reprod. 1995;10(Suppl 2):43–54
  42. Simonart T, Van Vooren JP. Interleukin-1 beta increases the BCL-2/BAX ratio in Kaposi's sarcoma cells. Cytokine. 2002;19:259–266
  43. Surrey ES, Halme J. Effect of peritoneal fluid from endometriosis patients on endometrial stromal cell proliferation in vitro. Obstet. Gynecol. 1990;76:792–797
  44. Wang Y, Matsuo H, Kurachi O, Maruo T. Down-regulation of proliferation and up-regulation of apoptosis by gonadotropin-releasing hormone agonist in cultured uterine leiomyoma cells. Eur. J. Endocrinol. 2002;146:447–456
  45. Yang Z, Mo X, Gong Q, Pan Q, Yang X, Cai W, et al. Critical effect of VEGF in the process of endothelial cell apoptosis induced by high glucose. Apoptosis. 2008;13:1331–1343
  46. Youle RJ, Strasser A. The BCL-2 protein family: opposing activities that mediate cell death. Nat. Rev. Mol. Cell Biol. 2008;9:47–59

PII: S0165-0378(10)00007-0

doi: 10.1016/j.jri.2009.12.002

Journal of Reproductive Immunology
Volume 84, Issue 2 , Pages 193-198 , March 2010

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