Characterization of the invasive and inflammatory traits of oral Campylobacter rectus in a murine model of fetoplacental growth restriction and in trophoblast cultures

Arce, R.M.; Diaz, P.I.; Barros, S.P.; Galloway, P.; Bobetsis, Y.; Threadgill, D.; Offenbacher, S.

doi:10.1016/j.jri.2009.11.003

« Previous Next »Journal of Reproductive Immunology
Volume 84, Issue 2 , Pages 145-153, March 2010

Characterization of the invasive and inflammatory traits of oral Campylobacter rectus in a murine model of fetoplacental growth restriction and in trophoblast cultures

R.M. Arce
- Center for Oral and Systemic Diseases, NC Oral Health Institute, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
- Authors equally contributed to this report.
P.I. Diaz
- Department of Periodontology, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
- Authors equally contributed to this report.
- Current affiliation: Division of Periodontology, Department of Oral Health and Diagnostic Sciences, School of Dental Medicine, The University of Connecticut Health Center, Farmington, CT, USA.
S.P. Barros
- Center for Oral and Systemic Diseases, NC Oral Health Institute, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
- Department of Periodontology, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
P. Galloway
- Center for Oral and Systemic Diseases, NC Oral Health Institute, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Y. Bobetsis
- Center for Oral and Systemic Diseases, NC Oral Health Institute, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
- Department of Periodontology, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
- Current affiliation: Department of Periodontology, School of Dentistry, University of Athens, Athens, Greece.
D. Threadgill
- Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
- Current affiliation: Department of Microbiology, North Carolina State University, Raleigh, NC, USA.
S. Offenbacher
- Center for Oral and Systemic Diseases, NC Oral Health Institute, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
- Department of Periodontology, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
- Corresponding author at: NC Oral Health Institute, School of Dentistry, University of North Carolina at Chapel Hill, 79 T.W. Alexander Drive, 4301 Research Commons, Research Triangle Park, NC 27709, USA. Tel.: +1 919 425 3596; fax: +1 919 425 3532.

Received 3 September 2009; received in revised form 27 October 2009; accepted 23 November 2009. published online 30 December 2009.

Abstract

Campylobacter species (C. jejuni, C. fetus) are enteric abortifacient bacteria in humans and ungulates. Campylobacter rectus is a periodontal pathogen associated with human fetal exposure and adverse pregnancy outcomes including preterm delivery. Experiments in pregnant mice have demonstrated that C. rectus can translocate from a distant site of infection to the placenta to induce fetal growth restriction and impair placental development. However, placental tissues from human, small-for-gestational age deliveries have not been reported to harbor C. rectus despite evidence of maternal infection and fetal exposure by fetal IgM response. This investigation examined the temporal relationship between the placental translocation of C. rectus and the effects on fetal growth in mice. BALB/c mice were infected at gestational day E7.5 to examine placental translocation of C. rectus by immunohistology. C. rectus significantly decreased fetoplacental weight at E14.5 and at E16.5. C. rectus was detected in 63% of placentas at E14.5, but not at E16.5. In in vitro trophoblast invasion assays, C. rectus was able to effectively invade human trophoblasts (BeWo) but not murine trophoblasts (SM9-1), and showed a trend for more invasiveness than C. jejuni. C. rectus challenge significantly upregulated both mRNA and protein levels of IL-6 and TNFα in a dose-dependent manner in human trophoblasts, but did not increase cytokine expression in murine cells, suggesting a correlation between invasion and cytokine activation. In conclusion, the trophoblast-invasive trait of C. rectus that appears limited to human trophoblasts may play a role in facilitating bacterial translocation and placental inflammation during early gestation.