Journal of Reproductive Immunology
Volume 84, Issue 1 , Pages 24-31 , January 2010

Identification of human sperm proteins that interact with human zona pellucida3 (ZP3) using yeast two-hybrid system

  • Rajesh K. Naz

      Affiliations

    • Corresponding Author InformationCorresponding author at: Robert C. Byrd Health Sciences Center North, Room 2084, West Virginia University, School of Medicine, Morgantown, WV 26506-9186, United States. Tel.: +1 304 293 2554; fax: +1 304 293 5757.
    • ,
  • Latha Dhandapani

Received 14 July 2009 ,Revised 30 September 2009 ,Accepted 10 October 2009.

References 

  1. Allen JB, Walberg MW, Edwards MC, Elledge SJ. Finding prospective partners in the library: the two-hybrid system and phage display find a match. Trends Biochem. Sci. 1995;20:511–516
  2. Bao L, Redondo C, Findlay JB, Walker JH, Ponnambalam S. Deciphering soluble and membrane protein function using yeast systems. Mol. Membr. Biol. 2009;26:127–135
  3. Chakravarty S, Suraj K, Gupta SK. Baculovirus expressed recombinant human zona pellucida glycoprotein-B induces acrosomal exocytosis in capacitated spermatozoa in addition to zona pellucida glycoprotein C. Mol. Hum. Reprod. 2005;11:365–372
  4. Chapman NR, Kessopoulou E, Andrews P, Hornby D, Barratt CR. The polypeptide backbone of recombinant human zona pellucida glycoprotein-3 initiates acrosomal exocytosis in human spermatozoa in vitro. Biochem. J. 1998;330:839–845
  5. Dean J. The enigma of sperm–egg recognition in mice. In:  Gupta SK,  Koyama K,  Murray FF editor. Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Nottingham, United Kingdom: Nottingham University Press; 2006;p. 359–365
  6. Fields S, Sternglanz R. The two-hybrid system: an assay for protein–protein interactions. Trends Genet. 1994;10:286–292
  7. Guarente L. Strategies for the identification of interacting proteins. Proc. Natl. Acad. Sci. U.S.A. 1993;90:1639–1641
  8. Gupta SK, Chakravarty S, Suraj K, Bansal P, Ganguly A, Jain MK, et al. Structural and functional attributes of zona pellucida glycoproteins. In:  Gupta SK,  Koyama K,  Murray FF editor. Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Nottingham, United Kingdom: Nottingham University Press; 2006;p. 203–216
  9. Harris JD, Hibler DW, Fontenot GK, Hsu KT, Yurewicz EC, Sacco AG. Cloning and characterization of zona pellucida genes and cDNAs from a variety of mammalian species: the ZPA, ZPB and ZPC gene families. DNA Seq. 1994;4:361–393
  10. Harris JD, Seid CA, Fontenot GK, Liu HF. Expression and purification of recombinant human zona pellucida proteins. Protein Express. Purif. 1999;16:298–307
  11. Kadam AL, Fateh M, Naz RK. Fertilization antigen (FA-1) completely blocks human sperm binding to human zona pellucida: FA-1 antigen may be a sperm receptor for zona pellucida in humans. J. Reprod. Immunol. 1995;29:19–30
  12. Yi Y, Manandhar G, Oko RJ, Breed WG, Sutovsky P. Mechanism of sperm–zona pellucida penetration during mammalian fertilization. In:  Gupta SK,  Koyama K,  Murray FF editor. Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Nottingham, United Kingdom: Nottingham University Press; 2006;p. 385–408
  13. Mandal A, Klotz KL, Shetty J, Jayes FL, Wolkowicz MJ, Bolling LC, et al. SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa. Biol. Reprod. 2003;68:1525–1537
  14. Naz RK, Ahmad K. Molecular identities of human sperm proteins that bind human zona pellucida: nature of sperm–zona interaction, tyrosine kinase activity, and involvement of FA-1. Mol. Reprod. Dev. 1994;39:397–408
  15. Naz RK, Chauhan SC. Human sperm-specific peptide vaccine that causes long term reversible contraception. Biol. Reprod. 2002;67:674–680
  16. Naz RK, Zhu X, Kadam AL. Identification of human sperm peptide sequence involved in egg binding for immunocontraception. Biol. Reprod. 2000;62:318–324
  17. Parmentier M, Libert F, Schurmans S, Schiffmann S, Lefort A, Eggerickx D, et al Expression of members of the putative olfactory receptor gene family in mammalian germ cells. Nature. 1992;355:453–455
  18. Sakai N, Sawada MT, Sawada H. Non-traditional roles of ubiquitin-proteasome system in fertilization and gametogenesis. Int. J. Biochem. Cell Biol. 2004;36:776–784
  19. Samuel AS, Naz RK. Isolation of human single chain variable fragment antibodies against specific sperm antigens for immunocontraceptive development. Hum. Reprod. 2008;23:1324–1337
  20. Spehr M, Hatt H. hOR17-4 as a potential therapeutic target. Drug News Perspect. 2004;17:165–171
  21. Suter B, Kittanakom S, Stagljar I. Two-hybrid technologies in proteomics research. Curr. Opin. Biotechnol. 2008;19:316–323
  22. Sutovsky P, Moreno RD, Ramalho-Santos J, Dominko T, Simerly C, Schatten G. Ubiquitin tag for sperm mitochondria. Nature. 1999;402:371–372
  23. Sutovsky P, Manadhar G, McCauley TC, Caamano JN, Sutovsky M, Thompson WE, et al. Proteasomal interference prevents zona pellucida penetration and fertilization in mammals. Biol. Reprod. 2004;71:1625–1637
  24. Tulsiani DRP, Zeng H, Abou-Haila A. Biology of sperm capacitation: evidence for multiple signaling pathways. In:  Gupta SK,  Koyama K,  Murray FF editor. Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Nottingham, United Kingdom: Nottingham University Press; 2006;p. 257–272
  25. Vanderhaeghen P, Schurmans S, Vassart G, Parmentier M. Olfactory receptors are displayed on dog mature sperm cells. J. Cell Biol. 1993;123:1441–1452
  26. Vosshall LB. Olfaction: attracting both sperm and the nose. Curr. Biol. 2004;14:918–920
  27. Xu B, Hao Z, Jha JN, Digilio L, Urekar C, Kim YH, et al. Validation of a testis specific serine/threonine kinase [TSSK] family and the substrate of TSSK1 &2, TSKS, as contraceptive targets. In:  Gupta SK,  Koyama K,  Murray FF editor. Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Nottingham, United Kingdom: Nottingham University Press; 2006;p. 87–101
  28. Yagi T. Clustered protocadherin family. Dev. Growth Differ. 2008;50:S131–140
  29. Yamamura R, Nishimura N, Nakatsuji H, Arase S, Sasaki T. The interaction of JRAB/MIKAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions. Mol. Biol. Cell. 2007;19:971–983
  30. Yanagimachi R. Mammalian fertilization. In:  Knobil E,  Neil JD editor. Physiology of Reproduction. New York: Raven Press; 1994;p. 189–317

PII: S0165-0378(09)00524-5

doi: 10.1016/j.jri.2009.10.006

Journal of Reproductive Immunology
Volume 84, Issue 1 , Pages 24-31 , January 2010

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