Journal of Reproductive Immunology
Volume 84, Issue 1 , Pages 100-110 , January 2010

Placental blood leukocytes are functional and phenotypically different than peripheral leukocytes during human labor

  • Rodrigo Vega-Sanchez

      Affiliations

    • Research Direction, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
    • RVS is a student of the Doctorate in Biomedical Sciences at the School of Medicine, Universidad Nacional Autonoma de Mexico (UNAM), and was supported by Scholarships 181447 from CONACyT and 504006841 from DGEP-UNAM and grant SALUD 2007-69353 from CONACyT.
    • ,
  • Nardhy Gomez-Lopez

      Affiliations

    • Research Direction, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
    • ,
  • Arturo Flores-Pliego

      Affiliations

    • Research Direction, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
    • ,
  • Susana Clemente-Galvan

      Affiliations

    • Research Direction, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
    • ,
  • Guadalupe Estrada-Gutierrez

      Affiliations

    • Research Direction, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
    • ,
  • Alejandro Zentella-Dehesa

      Affiliations

    • Department of Biochemistry, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Mexico City, Mexico
    • Department of Genomic Medicine and Environmental Toxicology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico
    • ,
  • Rolando Maida-Claros

      Affiliations

    • Research Direction, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
    • ,
  • Jorge Beltran-Montoya

      Affiliations

    • Research Direction, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
    • ,
  • Felipe Vadillo-Ortega

      Affiliations

    • Department of Experimental Medicine, School of Medicine, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico
    • Corresponding Author InformationCorresponding author at: Department of Experimental Medicine, School of Medicine, Universidad Nacional Autonoma de Mexico, Mexico D.F., Dr. Balmis 148, Col. Doctores, Mexico, D.F. 06720, Mexico. Tel.: +52 55 56232663.

Received 18 March 2009 ,Revised 22 July 2009 ,Accepted 18 August 2009.

References 

  1. Parry S, Strauss JF. Premature rupture of the fetal membranes. N. Engl. J. Med. 1998;338(10):663–670
  2. Vadillo-Ortega F, Hernandez A, Gonzalez-Avila G, Bermejo L, Iwata K, Strauss JF. Increased matrix metalloproteinase activity and reduced tissue inhibitor of metalloproteinases-1 levels in amniotic fluids from pregnancies complicated by premature rupture of membranes. Am. J. Obstet. Gynecol. 1996;174(4):1371–1376
  3. Estrada Gutierrez G, Vadillo-Ortega F, Jimenez Gonzalez MA, Beltran Montoya J, Giono Cerezo S. Caracterizacion bioquimica y ultraestructural del efecto de la interleucina 1-beta como mediador de degradacion de tejido conectivo en membranas corioamnioticas humanas en cultivo. Ginecol. Obstet. Mex. 2002;70:190–195
  4. Arechavaleta-Velasco F, Ogando D, Parry S, Vadillo-Ortega F. Production of matrix metalloproteinase-9 in lipopolysaccharide-stimulated human amnion occurs through an autocrine and paracrine proinflammatory cytokine-dependent system. Biol. Reprod. 2002;67(6):1952–1958
  5. Osman I, Young A, Ledingham MA, Thomson AJ, Jordan F, Greer IA, et al. Leukocyte density and pro-inflammatory cytokine expression in human fetal membranes, decidua, cervix and myometrium before and during labour at term. Mol. Hum. Reprod. 2003;9(1):41–45
  6. Thomson AJ, Telfer JF, Young A, Campbell S, Stewart CJ, Cameron IT, et al. Leukocytes infiltrate the myometrium during human parturition: further evidence that labour is an inflammatory process. Hum. Reprod. 1999;14(1):229–236
  7. Winkler M, Fischer DC, Ruck P, Marx T, Kaiserling E, Oberpichler A, et al. Parturition at term: parallel increases in interleukin-8 and proteinase concentrations and neutrophil count in the lower uterine segment. Hum. Reprod. 1999;14(4):1096–1100
  8. Menon R, Swan KF, Lyden TW, Rote NS, Fortunato SJ. Expression of inflammatory cytokines (interleukin-1 beta and interleukin-6) in amniochorionic membranes. Am. J. Obstet. Gynecol. 1995;172(2 Pt 1):493–500
  9. Keelan JA, Marvin KW, Sato TA, Coleman M, McCowan LM, Mitchell MD. Cytokine abundance in placental tissues: evidence of inflammatory activation in gestational membranes with term and preterm parturition. Am. J. Obstet. Gynecol. 1999;181(6):1530–1536
  10. Vadillo-Ortega F, Gonzalez-Avila G, Furth EE, Lei H, Muschel RJ, Stetler-Stevenson WG, et al. 92-kd type IV collagenase (matrix metalloproteinase-9) activity in human amniochorion increases with labor. Am. J. Pathol. 1995;146(1):148–156
  11. Mitchell MD, Trautman MS, Dudley DJ. Cytokine networking in the placenta. Placenta. 1993;14(3):249–275
  12. Peltier MR. Immunology of term and preterm labor. Reprod. Biol. Endocrinol. 2003;1:122
  13. Bulmer JN, Sunderland CA. Immunohistological characterization of lymphoid cell populations in the early human placental bed. Immunology. 1984;52(2):349–357
  14. Bokstrom H, Brannstrom M, Alexandersson M, Norstrom A. Leukocyte subpopulations in the human uterine cervical stroma at early and term pregnancy. Hum. Reprod. 1997;12(3):586–590
  15. Young A, Thomson AJ, Ledingham M, Jordan F, Greer IA, Norman JE. Immunolocalization of proinflammatory cytokines in myometrium, cervix, and fetal membranes during human parturition at term. Biol. Reprod. 2002;66(2):445–449
  16. Gomez-Lopez N, Estrada-Gutierrez G, Jimenez-Zamudio L, Vega-Sanchez R, Vadillo-Ortega F. Fetal membranes exhibit selective leukocyte chemotaxic activity during human labor. J. Reprod. Immunol. 2009;80(1):122–131
  17. Lopez-Bojorquez LN, Arechavaleta-Velasco F, Vadillo-Ortega F, Montes-Sanchez D, Ventura-Gallegos JL, Zentella-Dehesa A. NF-kappaB translocation and endothelial cell activation is potentiated by macrophage-released signals co-secreted with TNF-alpha and IL-1beta. Inflamm. Res. 2004;53(10):567–575
  18. Tachado SD, Zhang J, Zhu J, Patel N, Koziel H. HIV impairs TNF-alpha release in response to Toll-like receptor 4 stimulation in human macrophages in vitro. Am. J. Respir. Cell Mol. Biol. 2005;33(6):610–621
  19. Moore JM, Shi YP, Othoro C, Nahlen BL, Lal AA, Udhayakumar V. Comparative flow cytometric analysis of term placental intervillous and peripheral blood from immediate postpartum women in Western Kenya. Placenta. 2003;24(7):779–785
  20. Gomez Lopez NY, Estrada-Gutierrez G, Vadillo-Perez L, Vadillo-Ortega F. Changes in leukocyte subpopulations in the choriodecidua and fetal membranes during human labor. Reprod. Sci. 2008;15(2 Suppl.):86A
  21. Steel JH, O’Donoghue K, Kennea NL, Sullivan MH, Edwards AD. Maternal origin of inflammatory leukocytes in preterm fetal membranes, shown by fluorescence in situ hybridisation. Placenta. 2005;26(8–9):672–677
  22. Cindrova-Davies T, Hong-Wa Y, Johns J, Spasic-Boskovic O, Korolchuk S, Jauniaux E, et al. Oxidative stress, gene expression, and protein changes induced in the human placenta during labor. Am. J. Pathol. 2007;171(4):1168–1179
  23. Vadillo-Ortega F, Sadowsky DW, Haluska GJ, Hernandez-Guerrero C, Guevara-Silva R, Gravett MG, et al. Identification of matrix metalloproteinase-9 in amniotic fluid and amniochorion in spontaneous labor and after experimental intrauterine infection or interleukin-1 beta infusion in pregnant rhesus monkeys. Am. J. Obstet. Gynecol. 2002;186(1):128–138
  24. Stygar D, Wang H, Vladic YS, Ekman G, Eriksson H, Sahlin L. Increased level of matrix metalloproteinases 2 and 9 in the ripening process of the human cervix. Biol. Reprod. 2002;67(3):889–894
  25. Choi SJ, Oh S, Kim JH, Roh CR. Changes of nuclear factor kappa B (NF-kappaB), cyclooxygenase-2 (COX-2) and matrix metalloproteinase-9 (MMP-9) in human myometrium before and during term labor. Eur. J. Obstet. Gynecol. Reprod. Biol. 2007;132(2):182–188
  26. Garcia-Lopez G, Vadillo-Ortega F, Merchant-Larios H, Maida-Claros R, Osorio M, Soriano-Becerril D, et al. Evidence of in vitro differential secretion of 72 and 92kDa type IV collagenases after selective exposure to lipopolysaccharide in human fetal membranes. Mol. Hum. Reprod. 2007;13(6):409–418
  27. Zaga-Clavellina V, Merchant-Larios H, Garcia-Lopez G, Maida-Claros R, Vadillo-Ortega F. Differential secretion of matrix metalloproteinase-2 and -9 after selective infection with group B streptococci in human fetal membranes. J. Soc. Gynecol. Investig. 2006;13(4):271–279
  28. Vega-Sanchez R, Flores A, Castillo M, Gomez N, Vadillo-Ortega F. Expression, tissue traffic and activation of MMP-9 in human fetal membranes during labor. Reprod. Sci. 2008;15(1):164

PII: S0165-0378(09)00411-2

doi: 10.1016/j.jri.2009.08.002

Journal of Reproductive Immunology
Volume 84, Issue 1 , Pages 100-110 , January 2010

Article Tools

* Image Usage & Resolution