Journal of Reproductive Immunology
Volume 78, Issue 2 , Pages 115-124 , July 2008

Fusion of hC3d3 to hCGβ enhances responsiveness in vitro of human peripheral immunocompetent cells upon the antigen primary challenge

  • Hua-Ping Li

      Affiliations

    • Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Fudan University Shanghai Medical College, Shanghai 200011, China
    • Department of Obstetrics & Gynecology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China
    • These authors contributed equally to this work.
    • ,
  • Xiao-Ju He

      Affiliations

    • Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Fudan University Shanghai Medical College, Shanghai 200011, China
    • Shanghai Changning Maternity & Infant Health Institute, Shanghai 200052, China
    • These authors contributed equally to this work.
    • ,
  • Chuan-ling Tang

      Affiliations

    • Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Fudan University Shanghai Medical College, Shanghai 200011, China
    • ,
  • Xiao-Ying Yao

      Affiliations

    • Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Fudan University Shanghai Medical College, Shanghai 200011, China
    • ,
  • Da-Jin Li

      Affiliations

    • Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Fudan University Shanghai Medical College, Shanghai 200011, China
    • Department of Obstetrics & Gynecology, The Affiliated Hospital, Hainan Medical College, Haikou 570102, China
    • Corresponding Author InformationCorresponding author at: Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Fudan University Shanghai Medical College, Shanghai 200011, China. Tel.: +86 21 63457331/55050x386; fax: +86 21 63457331.

Received 8 February 2007 ,Revised 17 January 2008 ,Accepted 7 March 2008.

References 

  1. Banki Z, Kacani L, Rusert P, Pruenster M, Wilflingseder D, Falkensammer B, et al. Complement dependent trapping of infectious HIV in human lymphoid tissues. AIDS. 2005;19:481–486
  2. Breukels MA, Zandvoort A, Rijkers GT, Lodewijk ME, Klok PA, Harms G, et al. Complement dependency of splenic localization of pneumococcal polysaccharide and conjugate vaccines. Scand. J. Immunol. 2005;61:322–328
  3. Carroll MC. The role of complement in B cell activation and tolerance. Adv. Immunol. 2000;74:61–88
  4. Carter RH, Fearon DT. CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 1992;256:105–107
  5. Carter RH, Spycher MO, Ng YC, Hoffman R, Fearon DT. Synergistic interaction between complement receptor type 2 and membrane IgM on B lymphocytes. J. Immunol. 1988;141:457–463
  6. Cekan SZ, Aedo AR. Assays of antibodies to a C-terminal peptide or the entire β-subunit of human chorionic gonadotropin. J. Clin. Lab. Anal. 1998;12:60–64
  7. Cherukuri A, Cheng PC, Sohn HW, Pierce SK. The CD19/CD21 complex functions to prolong B cell antigen receptor signaling from lipid rafts. Immunity. 2001;14:169–179
  8. Dempsey PW, Allison MED, Akkaraju S, Goodnow CC, Fearon DT. C3d of complement as a molecular adjuvant: bridging innate and acquired immunity. Science. 1996;271:348–350
  9. Fakher M, Wu J, Qin D, Szakal A, Tew J. Follicular dendritic cell accessory activity crosses MHC and species barriers. Eur. J. Immunol. 2001;31:176–185
  10. Fingeroth JD, Benedict MA, Levy DN, Strominger JL. Identification of murine complement receptor type 2. Proc. Natl. Acad. Sci. U.S.A. 1989;86:242–246
  11. Fischer MB, Goerg S, Shen L, Prodeus AP, Goodnow CC, Kelsoe G, et al. Dependence of germinal center B cells on expression of CD21/CD35 for survival. Science. 1998;280:582–585
  12. Green TD, Newton BR, Rota PA, Xu Y, Robinson HL, Ross TM. C3d enhancement of neutralizing antibodies to measles hemagglutinin. Vaccine. 2002;20:242–248
  13. Green TD, Montefiori DC, Ross TM. Enhancement of antibodies to the human immunodeficiency virus type 1 envelope by using the molecular adjuvant C3d. J. Virol. 2003;77:2046–2055
  14. Haas KM, Toapanta FR, Oliver JA, Poe JC, Weis JH, Karp DR, et al. Cutting edge: C3d functions as a molecular adjuvant in the absence of CD21/35 expression. J. Immunol. 2004;172:5833–5837
  15. Kozono Y, Abe R, Kozono H, Kelly RG, Azuma T, Holers VM. Cross-linking CD21/CD35 or CD19 increases both B7-1 and B7-2 expression on murine splenic B cells. J. Immunol. 1998;160:1565–1672
  16. Li DJ, Wang HM, Li L, Zhao XR, Wang MY, Zhu Y, et al. Gene fusion of molecular adjuvant C3d to hCGβ enhances the anti-hCGβ antibody response in DNA immunization. J. Reprod. Immunol. 2003;60:129–141
  17. Lyubchenko T, Porto DJ, Cambier JC, Holers VM. Coligation of the B cell receptor with complement receptor type 2 (CR2/CD21) using its natural ligand C3dg: activation without engagement of an inhibitory signaling pathway. J. Immunol. 2005;174:3264–3272
  18. O’Rand MG, Lea IA. Designing an effective immunocontraceptive. J. Reprod. Immunol. 1997;36:51–59
  19. Pierce SK. Lipid rafts and B-cell activation. Nat. Rev. Immunol. 2002;2:96–105
  20. Rose NR. Immunological hazards associated with vaccination of humans. J. Autoimmun. 2000;14:11–13
  21. Ross TM, Xu Y, Bright RA, Robinson HL. C3d enhancement of antibodies to hemagglutinin accelerates protection against influenza virus challenge. Nat. Immunol. 2000;1:127–131
  22. Stevens VC. Antifertility vaccines. In:  Perlmann P,  Wigzell H editor. Handbook of Experimental Pharmacology. Springer-Verlag; 1999;p. 443–461
  23. Talwar GP, Singh OM, Pal R, Chatterjee N, SahaI P, Dhall K, et al. A vaccine that prevents pregnancy in women. Proc. Natl. Acad. Sci. U.S.A. 1994;91:8532–8536
  24. Talwar GP. Vaccines and passive immunological approaches for the control of fertility and hormone-dependent cancers. Immunol. Rev. 1999;171:173–192
  25. Terrazzini N, Hannesdottir S, Delves PJ, Lund T. DNA immunization with plasmids expressing hCGbeta-chimeras. Vaccine. 2004;22:2146–2153
  26. Wang XL, Li DJ, Yuan MM, Yu M, Yao XY. Enhancement of humoral immunity to the hCG beta protein antigen by fusing a molecular adjuvant C3d3. J. Reprod. Immunol. 2004;63:97–110
  27. Wang XL, Zhao XR, Yu M, Yuan MM, Yao XY, Li DJ. Gene conjugation of molecular adjuvant C3d3 to hCGβ increased the anti-hCGβ Th2 and humoral immune response in DNA immunization. J. Gene Med. 2006;8:498–505
  28. Yu Min, Li DJ, Wang XL, Zhao XR, Yao XY. Molecular adjuvant C3d up-regulates both B7-1 and B7-2 expression on Raji cells. J. Mol. Cell. Biol. 2006;39:77–82

PII: S0165-0378(08)00028-4

doi: 10.1016/j.jri.2008.03.004

Journal of Reproductive Immunology
Volume 78, Issue 2 , Pages 115-124 , July 2008

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