Journal of Reproductive Immunology
Volume 78, Issue 2 , Pages 94-101 , July 2008

Production and characterization of a novel monoclonal antibody against progesterone-induced blocking factor (PIBF)

  • Ekaterina Ivanova-Todorova

      Affiliations

    • Department of Molecular Immunology, Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, Bulgaria
    • ,
  • Dobroslav S. Kyurkchiev

      Affiliations

    • Department of Clinical Laboratory and Clinical Immunology, University Hospital ‘St.I.Rilski’, Medical University Sofia, Bulgaria
    • ,
  • Angel Nalbanski

      Affiliations

    • University Hospital of Obstetrics and Gynecology ‘Maichin dom’, Sofia, Bulgaria
    • ,
  • Tanya Timeva

      Affiliations

    • Ob/Gyn Hospital ‘Dr Shterev’, Sofia, Bulgaria
    • ,
  • Atanas Shterev

      Affiliations

    • Ob/Gyn Hospital ‘Dr Shterev’, Sofia, Bulgaria
    • ,
  • Stanimir D. Kyurkchiev

      Affiliations

    • Department of Molecular Immunology, Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, Bulgaria
    • Corresponding Author InformationCorresponding author at: Department of Molecular Immunology, Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, 73 Tzarigradsko shosse, 1113 Sofia, Bulgaria. Tel.: +359 2 723890; fax: +359 2 720925.

Received 4 September 2007 ,Revised 5 December 2007 ,Accepted 11 December 2007.

References 

  1. Check J, Szekeres-Bartho J, O'Slaughnessy A. Progesterone induced blocking factor seen in pregnancy lymphocytes soon after implantation. Am. J. Reprod. Immunol. 1996;35:277–280
  2. Check K, Nazari P, Goldberg J, et al. A model for potential tumor immunotherapy based on knowledge of immune mechanisms responsible for spontaneous abortion. Med. Hypotheses. 2001;57:337–343
  3. Kozma N, Halasz M, Polgar B, et al. Progesterone-induced blocking factor activates STAT6 via binding to a novel IL-4 receptor. J. Immunol. 2006;176:819–826
  4. Kyurkchiev S, Surneva-Nakova Ts, Ivanova M, et al. Monoclonal antibodies to porcine zona pellucida that block the initial stages of fertilization. Am. J. Reprod. Immunol. Microbiol. 1988;18:11–18
  5. Lachmann M, Gelbmann D, Kálmán E, Polgár , et al. PIBF (progesterone induced blocking factor) is overexpressed in highly proliferating cells and associated with the centrosome. Int. J. Cancer. 2004;112:51–60
  6. Polgar B, Barakonyi A, Xynos I, Szekeres-Bartho J. The role of γδ TCR positive cells in pregnancy. Am. J. Reprod. Immunol. 1999;41:239–244
  7. Polgar B, Kispal G, Lachmann M, et al. Molecular cloning and immunologic characterization of a novel cDNA coding for progesterone-induced blocking factor. J. Immunol. 2003;171:5956–5963
  8. Polgar B, Nagy E, Miko E. Urinary progesterone-induced blocking factor concentration is related to pregnancy outcome. Biol. Reprod. 2004;71:1699–1705
  9. Srivastava MD, Check JH, Szekeres-Bartho J. Expression of progesterone-induced blocking factor (PIBF) by human cell lines. J. Clin. Oncol. 2004;22:3191;July 15 Supplement (abstract)
  10. Szekeres-Bartho J, Kilar F, Falkay G, et al. The mechanism of the inhibitory effect of progesterone on lymphocyte cytotoxicity. I. Progesterone-treated lymphocytes release a substance inhibiting cytotoxicity and prostaglandin synthesis. Am. J. Reprod Immunol. Microbiol. 1985;9:15–18
  11. Szekeres-Bartho J, Autran B, Debre P, et al. Immunoregulatory effects of a suppressor factor from healthy pregnant women's lymphocytes after progesterone induction. Cell. Immunol. 1989;122:281–294
  12. Szekeres-Bartho J, Varga P, Pejtsik B. ELISA test for the detection of an immunological blocking factor in human pregnancy serum. J. Reprod. Immunol. 1989;16:19–29
  13. Szekeres-Bartho J, Wegmann TG. A progesterone-dependent immunomodulatory protein alters the Th1/Th2 balance. J. Reprod. Immunol. 1996;31:81–95
  14. Szekeres-Bartho J, Faust Z, Varga P, et al. The immunological pregnancy protective effect of progesterone is manifested via controlling cytokine production. Am. J. Reprod. Immunol. 1996;35:348–351
  15. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 1979;76:4350–4356

PII: S0165-0378(07)00271-9

doi: 10.1016/j.jri.2007.12.001

Journal of Reproductive Immunology
Volume 78, Issue 2 , Pages 94-101 , July 2008

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