Anti-apoptotic seminal vesicle protein IV inhibits cell-mediated immunity

Fuggetta, M.P.; Lanzilli, G.; Cottarelli, A.; Ravagnan, G.; Cartenì, M.; De Maria, S.; Metafora, B.M.; Metafora, V.; Metafora, S.

doi:10.1016/j.jri.2007.11.002

« Previous Next »Journal of Reproductive Immunology
Volume 78, Issue 2 , Pages 85-93, July 2008

Anti-apoptotic seminal vesicle protein IV inhibits cell-mediated immunity

M.P. Fuggetta
- Institute of Neurobiology and Molecular Medicine (INMM), CNR, Area Ricerca Roma ’Tor Vergata, Via del Fosso del Cavaliere 100, I-00133 Rome, Italy
G. Lanzilli
- Institute of Neurobiology and Molecular Medicine (INMM), CNR, Area Ricerca Roma ’Tor Vergata, Via del Fosso del Cavaliere 100, I-00133 Rome, Italy
A. Cottarelli
- Institute of Neurobiology and Molecular Medicine (INMM), CNR, Area Ricerca Roma ’Tor Vergata, Via del Fosso del Cavaliere 100, I-00133 Rome, Italy
G. Ravagnan
- Department of Environmental Sciences, Ca’Foscari Venezia University, Calle Larga Santa Marta, Venice, Italy
M. Cartenì
- Department of Experimental Pharmacology, Medical School, II University of Naples (SUN), Via De Crecchio 7, 80138 Naples, Italy
S. De Maria
- Department of Experimental Pharmacology, Medical School, II University of Naples (SUN), Via De Crecchio 7, 80138 Naples, Italy
B.M. Metafora
- Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, CNR, Via Pietro Castellino 111, 80128 Naples, Italy
V. Metafora
- Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, CNR, Via Pietro Castellino 111, 80128 Naples, Italy
S. Metafora
- Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, CNR, Via Pietro Castellino 111, 80128 Naples, Italy
- Corresponding author at: CNR Institute of Genetics and Biophysics ‘Adriano Buzzati Traverso’, Via Pietro Castellino 111, 80128 Naples, Italy. Tel.: +39 081 6132 254; fax: +39 081 6132 253.

Received 18 September 2007; received in revised form 30 October 2007; accepted 13 November 2007. published online 13 February 2008.

Abstract

The in vitro effect of seminal vesicle protein IV (SV-IV) on the cytotoxic activity of human natural or acquired cellular immunity has been investigated by standard immunological procedures, a ⁵¹Cr-release cytotoxicity assay, and labeled-ligand binding experiments. The data obtained demonstrate that: (1) fluoresceinated or [¹²⁵I]-labeled SV-IV binds specifically to the surface of human purified non-adherent monuclear cells (NA-MNC); (2) SV-IV suppresses the cytotoxicity of natural killer (NK) cells against K562 target cells, that of IL-2-stimulated NK (LAK) cells against DAUDI target cells, and that of VEL antigen-sensitized cytotoxic T lymphocytes (CTLs) against VEL target cells; (3) treatment of K562 target cells alone with SV-IV decreases their susceptibility to NK-induced lysis. These findings indicate that the protein SV-IV has a marked in vitro inhibitory effect on NK, LAK and CTL cytotoxicity, providing a better understanding of its immune regulatory functions.

Keywords: Protein SV-IV, NK, LAK, CTL, Immunomodulation, Binding experiments